human igfbp-3 duoset elisa  (Bio-Techne corporation)

Journal: Investigative Ophthalmology & Visual Science

Article Title: IGFBP-3 Mediates Metabolic Homeostasis During Hyperosmolar Stress in the Corneal Epithelium

doi: 10.1167/iovs.62.7.11

Figure Lengend Snippet: IGFBP-3 blocks the hyperosmolarity-induced decrease in mitochondrial respiration and glycolysis in corneal epithelial cells. The hTCEpi cells were cultured in basal media with or without hyperosmolar conditions for 24 hours. Cells cultured in 450 mOsM were cotreated with 500 ng/mL rhIGFBP-3. ( A ) IGFBP-3 secretion was down regulated in hyperosmolar culture in basal media ( * P < 0.001, one-way ANOVA, SNK multiple comparison test, n = 3). ( B ) Immunoblotting for intracellular IGFBP-3 in whole cell lysates. Consistent with the decrease in secreted IGFBP-3, intracellular IGFBP-3 was also sequentially decreased with increasing levels of salt. Beta-actin was used as a loading control. ( C ) Consistent with our prior results, both respiration and glycolysis were significantly decreased after culture in 450 mOsM/kg ( ** P = 0.021 and *** P = 0.011, one-way ANOVA, SNK multiple comparison test, n = 3). Treatment with rhIGFBP-3 restored metabolism and glycolysis to basal levels ( **** P = 0.042 and ***** P = 0.012, one-way ANOVA, SNK multiple comparison test, n = 3). ( D ) Hyperosmolar culture shifted cells toward a respiratory phenotype in hyperosmolar culture that was restored following treatment with rhIGFBP-3 ( ****** P = 0.002 and * P < 0.001, one-way ANOVA, SNK multiple comparison test, n = 3). ( E ) SYBR green staining for mitochondrial DNA showed a decrease in mtDNA after hyperosmolar culture that was blocked by co-treatment with rhIGFBP-3. Scale bar : 10 µm. Number indicates mOsM. Images representative of three repeated experiments.

Article Snippet: A solid phase sandwich ELISA was used to quantify IGFBP-3 levels (human Quantikine ELISA; R&D Systems, Minneapolis, MN, USA).

Techniques: Cell Culture, Comparison, Western Blot, SYBR Green Assay, Staining

Journal: Investigative Ophthalmology & Visual Science

Article Title: IGFBP-3 Mediates Metabolic Homeostasis During Hyperosmolar Stress in the Corneal Epithelium

doi: 10.1167/iovs.62.7.11

Figure Lengend Snippet: Hyperosmolar culture induced expression of the anti-inflammatory cytokines, IL-6 and IL-8. hTCEpi cells were cultured in basal media in isotonic and hyperosmolar conditions with and without 500 ng/mL rhIGFBP-3 for 24 hours. ( A ) Hyperosmolar culture triggered release of IL-6 that was not affected by treatment with rhIGFBP-3 ( * P = 0.008 for 450 compared to KBM, ** P = 0.004 for IGFBP-3 treated cells in hyperosmolar culture compared to KBM, one-way ANOVA, SNK multiple comparison test, n = 3). ( B ) IL-8 showed a more robust increase in hyperosmolar culture compared to KBM ( * P = 0.001). This increase was partially abrogated by treatment with rhIGFBP-3 ( ** P = 0.033, one-way ANOVA, SNK multiple comparison test). Data represented as mean ± standard deviation.

Article Snippet: A solid phase sandwich ELISA was used to quantify IGFBP-3 levels (human Quantikine ELISA; R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Cell Culture, Comparison, Standard Deviation

Journal: BMC Musculoskeletal Disorders

Article Title: Benefits of resistance exercise in lean women with fibromyalgia: involvement of IGF-1 and leptin

doi: 10.1186/s12891-017-1477-5

Figure Lengend Snippet: Serum levels of total IGF-1, serum free IGF-1, IGFBP3, adiponectin, leptin, and resistin at baseline, and change (∆) at posttest after resistance exercise

Article Snippet: Biological markers were analyzed by sandwich enzyme-linked immunosorbent assays (ELISAs) using a pair of specific antibodies for human adiponectin (DY1065, 62 pg/mL), human leptin (DY389, 31 pg/mL), human resistin (DY1359, 10 pg/mL), human serum free bioactive IGF-1 (DY291, 4 pg/mL) and IGFBP3 (DY675, 0.125 ng/ml) which were all purchased from RnD Systems (Minneapolis, MN, USA).

Techniques:

Journal: BMC Musculoskeletal Disorders

Article Title: Acute effects of physical exercise on the serum insulin-like growth factor system in women with fibromyalgia

doi: 10.1186/s12891-017-1402-y

Figure Lengend Snippet: Values obtained at high-intensity exercise performance in women with fibromyalgia (FM) ( n = 22) and the healthy reference group ( n = 27). Mean value and SD of differences (∆), p -value for within-group differences as well as for between- group differences are presented

Article Snippet: Total S-IGF-1 was measured by solid-phase, enzyme-labeled chemoluminescent immunoassay with IDS-iSYS IGF1 immunoassay (IS-3900, Immunodiagnostic Systems Boldon, UK), S-IGFBP-3 and free IGF-1 with reagents from DY675, RnD Systems, Minneapolis, MN, USA.

Techniques: